This film also goes by the alternate title
Rapunzel: A Tangled Tale, in my part of the world, at least.
There are three main human characters in this film, two of which are women: Rapunzel and her adoptive mother, Mother Gothel.
Despite the impression that some of the marketing material may have given, Rapunzel is very much the focus of the story. Her relationship with Mother Gothel is the driving force of the story's conflict, and their interactions lay central to the film.
( Character-related spoilers for the film. )That's the central characters.
There are a couple of supporting characters in the form of guards and ruffians that Rapunzel encounters, but none of them are women. She does cross paths with some women in the kingdom village, but none of them have dialogue.
( One more character-related spoiler. )